BcAMT1;5 Mediates Nitrogen Uptake and Assimilation in Flowering Chinese Cabbage and Improves Plant Growth When Overexpressed in Arabidopsis

Nitrogen (N) is a major limiting factor for plant growth and vegetable production. Understanding the regulatory mechanisms of N uptake, transport, assimilation key to improving use efficiency in plants. Ammonium transporters (AMTs) play an important role metabolism. In this study, we isolated AMT1 subfamily member (BcAMT1;5) with highly conserved signatural motif from flowering Chinese cabbage. Based on functional complementation yeast mutant 31019b overexpression BcAMT1;5 Arabidopsis, AMT. Tissue expression analysis showed that was mainly expressed roots multiple regime transcript patterns respond varying nutritional conditions. This up-regulated by N-deficiency down-regulated supplying NH4+. The glucuronidase (GUS) activities BcAMT1;5pro::GUS similar change response different Overexpression accelerated transgenic seedlings, increased NH4+ net influxes, enhanced content accumulation NO3− at low concentrations. Additionally, it levels assimilation-related genes shoots. These results indicate may participate uptake under various conditions cabbage, but differed obviously other AMT1s.